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Melanoma is a deadly disease that is largely incurable by chemotherapy and molecularly targeted therapy. Thus, the identification of novel and druggable molecular targets in melanoma cells is greatly needed. In the present study, we found that dabrafenib, an inhibitor of B-Raf kinase, effectively inhibits melanoma cell invasion and inhibits proliferation, migration, and invasion of melanoma cells. Intriguingly, dabrafenib treatment results in a significant increase of JNK-mediated phosphorylation of paxillin and decreases in Erk-mediated phosphorylation of paxillin in melanoma cells, suggesting that B-Raf kinase may be a mediator of paxillin phosphorylation and a potential regulator of melanoma cell migration and invasion. In addition, we found that knockdown of BRAF or inhibition of B-Raf kinase by GDC-0879 treatment resulted in significant loss of paxillin phosphorylation but decreases of paxillin phosphorylation were not observed in normal melanocytes. Our findings not only shed light on the mechanism by which B-Raf kinase regulation of paxillin phosphorylation affects melanoma cell migration and invasion but also may identify new druggable targets for the treatment of melanoma.Q: Test runner stopping (EnsureBrowser is running) when executing E2E tests with Protractor When executing my e2e tests through the protractor-cli.js, a pop-up appears and says: Failed: EnsureBrowser is running The scenario is: I'm executing a SpecRunner file through the protractor-cli.js The SpecRunner file executes all my e2e tests I'm using: Using an OS X machine Using VSCode 1.41.2 Using a connected VSCode remote runner When executing the SpecRunner file, the pop-up does not show: I've confirmed that the -i flag of protractor is pointing to the SpecRunner file How can I prevent this pop-up from appearing? A: If you want to avoid this, then change your manifest to include the browser-detector package: "browser-detector": { "config": "src/browser-detector.conf.json" }, What's New in the? In a plurality of video coding technologies, coding is performed by dividing a frame into blocks of a predetermined size and performing coding on each block. In the H.264 coding technology, intra prediction (prediction from a frame neighboring a block in a current frame) is performed on a current block. In addition, in H.264, an intra prediction mode can switch based on a flag value (a prediction direction flag value) indicating the direction of prediction (intra prediction). FIG. 12 illustrates a method of switching based on the prediction direction flag value in H.264. As illustrated in FIG. 12, in the case of switching between intra 4×4 and intra 16×16 modes, if the prediction direction flag value is 0, then intra 4×4 mode is selected. In the intra 16×16 mode, intra prediction in the vertical direction is switched to intra 4×4 prediction based on the prediction direction flag value. If the prediction direction flag value is 1, then intra 4×4 mode is selected for both vertical and horizontal directions. In this case, there is no need to switch prediction directions in both vertical and horizontal directions, and thus a number of switching operations is reduced. FIG. 13 illustrates a diagram of the intra prediction in a case where the prediction direction flag value is 0. As illustrated in FIG. 13, intra prediction is performed using a left prediction column and a top prediction column of a reference image of an intra 4×4 block on a left side of a current block. However, in the case where the prediction direction flag value is set to 1, as described above, horizontal and vertical prediction directions do not change between adjacent blocks, and thus there is a need for a coding apparatus to predict based on an equivalent intra prediction mode on a current block, to thereby reduce the number of prediction direction switching operations. Therefore, in H.264, intra prediction direction can switch in accordance with a prediction direction flag value, and a mode used when the prediction direction flag value is 1 is defined as an equivalent intra prediction mode (hereinafter referred to as an “equivalent intra prediction mode with no prediction direction flag value”). FIG. 14 illustrates a method of switching the prediction direction flag value. As illustrated in FIG. 14, a prediction direction flag value is set to 1 at positions where a block is predicted based on the equivalent intra prediction mode. Accordingly, a prediction direction flag value is set to 0 in a region other than the positions where a block System Requirements: CPU: Intel Dual-Core Core 2 Duo 2.13Ghz or better Intel Dual-Core Core 2 Duo 2.13Ghz or better RAM: 4GB or more (8GB recommended) 4GB or more (8GB recommended) Video card: Intel HD 3000, AMD 8500 or better Intel HD 3000, AMD 8500 or better HDD: 9.99GB available space (Additional 2GB required for installation) 9.99GB available space (Additional 2GB required for installation) Sound card: DirectX 9.0c compatible
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